Optically gated beating-heart imaging

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Optically gated beating-heart imaging

The constant motion of the beating heart presents an obstacle to clear optical imaging, especially 3D imaging, in small animals where direct optical imaging would otherwise be possible. Gating techniques exploit the periodic motion of the heart to computationally "freeze" this movement and overcome motion artifacts. Optically gated imaging represents a recent development of this, where image an...

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Self-gated 4D whole-heart imaging

Background Suppressing cardiac and respiratory motion artifacts are major challenges in cardiac MRI. The conventional methods of ECG and diaphragm navigator gating require tedious setup, reduce the imaging efficiency significantly, are susceptible to drifts in heart rate or respiratory pattern, and can be unreliable at higher field strengths [1]. In this work, we propose a fully self-gated 4D i...

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Real-time optical gating for three-dimensional beating heart imaging.

We demonstrate real-time microscope image gating to an arbitrary position in the cycle of the beating heart of a zebrafish embryo. We show how this can be used for high-precision prospective gating of fluorescence image slices of the moving heart. We also present initial results demonstrating the application of this technique to 3-D structural imaging of the beating embryonic heart.

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Intravital 2-photon imaging of leukocyte trafficking in beating heart.

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ژورنال

عنوان ژورنال: Frontiers in Physiology

سال: 2014

ISSN: 1664-042X

DOI: 10.3389/fphys.2014.00481